Micron-Txoj Kev Tiv Thaiv Lub Neej: Qhov Kev Ua Txhaum Cai Zoo Thiab Cov Txheej Txheem Txheej Txheem ntawm OPU Qe Retrieval Needle

Kev tsim ntawm OPU qe retrieval koob yog ib qho kev ua raws li cov kev txwv ntawm precision, huv si thiab kev ntseeg siab ntawm ib tug zoo heev scale. Lub raj hlau uas muaj txoj kab uas hla ntawm tsuas yog 1-2 millimeters thiab ntev tshaj 20 centimeters yuav tsum tau punctured los ntawm ntau lub zes qe menyuam follicles nyob rau hauv kev taw qhia ultrasound. Txawm tias qhov sib txawv me me hauv kev ua tau zoo tuaj yeem cuam tshuam rau kev cia siab ntawm tsev neeg. Rau cov chaw tsim khoom ntawm cov cuab yeej phais mob me me, ua tiav cov txheej txheem tsim khoom ntawm OPU koob yog cov ntawv pov thawj tsim nyog rau lawv nkag mus rau qib siab ntawm cov khoom siv kho mob qib siab. Cov txheej txheem kev teeb meem tshwj xeeb tshwm sim nyob rau hauv txhua yam ntawm cov ntaub ntawv, geometric raug thiab kho nto.
Cov khoom siv yog lub hauv paus ntawm kev ua haujlwm. Cov koob tshuaj OPU tseem ceeb yog ua los ntawm 316L kev kho mob stainless hlau lossis 5-qib titanium alloy. Stainless hlau muaj kev ua tau zoo tshaj plaws thiab cov nqi zoo; thaum titanium alloy, vim nws lub zog tshwj xeeb dua, biocompatibility zoo heev (txo cov cell adhesion), thiab ua tiav kev kho mob kev sib raug zoo, tau dhau los ua ib qho tseem ceeb ntawm cov khoom siab - kawg. Cov ntaub ntawv yuav tsum tau ua tiav - txheej txheem biocompatibility ntawv pov thawj los ntawm melting rau cov khoom tiav kom tsis muaj cytotoxicity. Kev txiav ntau dua- ntug kev tshawb fawb suav nrog kev siv cov txheej txheem ultra-lubricating hydrophilic rau ntawm cov phab ntsa sab hauv ntawm cov koob txhaj tshuaj kom txo tau cov ntaub so ntswg kev sib txhuam thiab cov adhesion poob ntawm oocytes hauv cov hlab.
Cov thev naus laus zis tseem ceeb tsom rau "kev ntse, smoothness thiab raug":
1. Kev ua tiav ntawm ultra- nyias thiab ntev- txoj kab uas hla: Cov khoom siv hlau yog rub mus rau hauv cov raj tsis tshua muaj nyias thiab ntev nrog ib txoj kab uas hla ntawm 1.2-1.6 hli, ib txoj kab uas hla ntawm kwv yees li 1 hli, thiab ntev tshaj 200 hli, thaum tuav tsis tshua muaj siab ncaj, roundness, thiab cov phab ntsa tuab. Qhov no yog qhov kev sib tw tseem ceeb ntawm kev tsim khoom, suav nrog ntau qhov dhau los ntawm cov duab kos thiab cov txheej txheem annealing.
2. Micro-metric shaping ntawm rab koob lub tswv yim thiab sab qhov: Lub kaum sab xis thiab lub ntsej muag ntawm rab koob yuav tsum tau ua tiav los ntawm kev sib tsoo kom ntseeg tau tias kev nkag mus tau zoo. Lub sab nqus qhov ntawm ob -chamber rab koob yog qhov nyuaj hauv cov txheej txheem, yuav tsum tau siv tshuab laser txiav los tsim cov qhov rais du thiab tsis muaj plaub hau nrog qhov nqaim heev (ntau kaum ntawm micrometers) ntawm phab ntsa ntawm lub koob raj. Qhov loj thiab txoj hauj lwm ntawm cov qhov no ncaj qha cuam tshuam rau qhov chaw nqus dej thiab qhov ua tau zoo ntawm lub qe ntes.
3. Kev ua haujlwm du ntawm cov kab noj hniav hauv cov kab noj hniav: Cov kab los ntawm cov qe thiab cov kua follicular yuav tsum tau ua kom du. Electrolytic polishing yog cov txheej txheem tseem ceeb, uas xaiv tshem tawm cov kab mob me me ntawm qhov chaw los ntawm cov txheej txheem electrochemical, ua tiav daim iav -zoo li smoothness ntawm phab ntsa sab hauv, uas yog ib qho tseem ceeb rau kev tiv thaiv cov cumulus nyuaj, txo cov cell puas, thiab tiv thaiv cov protein adhesion.
Tag nrho - txheej txheem plua plav- pub dawb thiab kev tswj kom huv yog lwm qhov cuam tshuam loj. OPU koob, uas los rau hauv kev sib cuag ncaj qha nrog gametes, yuav tsum tau tsim nyob rau hauv ib tug huv rhiav. Los ntawm kev ua ib feem, sib sau ua ke mus rau qhov kawg ntim, ntau cov txheej txheem tu ultrasonic nruj yuav tsum tau tshem tawm tag nrho cov khoom, roj, thiab cov khoom seem. Cov khoom kawg yuav tsum tau txais kev kho mob aseptic (feem ntau yog gamma irradiation sterilization) thiab kuaj endotoxin. Txhua yam kab mob me me tuaj yeem ua rau muaj qhov txawv txav ntawm lub embryo txoj kev loj hlob lossis lub voj voog tshem tawm.
Yog li ntawd, ib rab koob OPU uas tsim nyog yog kev sib xyaw ua ke ntawm cov khoom siv hlau, micro- ua haujlwm, nto engineering thiab kev tswj kom huv heev. Nws tsis tsuas yog ntsuas qhov tseeb ntawm cov cuab yeej siv tshuab, tab sis kuj tseem muaj cov txheej txheem tsim khoom lag luam thiab cov kab lis kev cai zoo raws li "cellular- qib kev nyab xeeb" tus qauv. Cov neeg tsim khoom lag luam uas tuaj yeem tsim cov khoom zoo li no ua pov thawj tias lawv muaj peev xwm ua haujlwm rau qhov xav tau tshaj plaws hauv kev tshawb fawb hauv lub neej, thiab tau ua txoj hauv kev rau lawv nkag mus rau lwm yam siab -tus nqi cell kho lossis cov cuab yeej kho noob lag luam.